Figure 1.
Characterization of LWW31. (A) Structures of LWW31 (from the top) in soluble form, LWW31 on beads, and LWW31 conjugated with linker-lysine-biotin (LKB). (B) Preliminary cytotoxicity assay for the 19 randomly chosen compounds at 40 μM. (C) LWW31 inhibited HepG2 cell proliferation with a lower IC50 than that observd for hHF cells. (D) LWW31-LKB pulls down EIF5B from both Jurkat (lane 4) and HepG2 (lane 9) cell lysates. Western blot: lane 1, total Jurkat cell lysate with mouse anti-EIF5B IgG; lane 2, Jurkat cell proteins pulled down with LKB; lane 3, Jurkat cell proteins pulled down with LWW29-LKB; lanes 5 and 6, molecular weight markers; lane 7, total HepG2 cell lysate with mouse anti-EIF5B; lane 8, HepG2 proteins pulled down with LKB. (E) LWW31 induced Jurkat cell apoptosis, whereas (F) LKB did not.