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LWW31 inhibited the incorporation of [35S]-methionine. (A) Phosphorimaging of SDS-PAGE resolved [35S]-methionine labeled proteins from cells treated with various concentrations of LWW31. (B) Coomassie blue staining of the SDS-PAGE used for phosphorimaging. (C) The ratio of [35S]-methionine-labeled protein radioactivity (CNT*mm2) to the amount of total protein (band density) of each lane on the same SDS-PAGE gel.
