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Effect of the sulfhydryl reagent DTNB on swarming of cells with Cys-substituted variants of PomB. Vibrio strain NMB192 (ΔpomB) harboring derivatives of pHK4 (pomB+) with Cys-substituted variants of pomB were spotted on semisolid plates containing no DTNB, 0.1 mM DTNB, and 0.5 mM DTNB and photographed after 4 h of incubation at 30°C. Relative swarm sizes were normalized to that of the wild type under the same conditions. The 31st residue is Cys in the wild-type PomB.
