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Combinations between Cys mutations in PomA TMs and PomB TM. Vibrio strain NMB191 (ΔpomAB) harboring derivatives of pYA303 (pomAB+) with Cys-substituted variants of pomA and pomB were spotted on semisolid plates containing 500 mM NaCl and 0 mM (A) or 2 mM (B) DTT and photographed after 4 h of incubation at 30°C. wt, wild type.
