TABLE 5.
Expression analysis of representative quorum sensing regulated genes
Gene | Fold change (microarray)a | Fold change (real-time PCR)b | Relevant comparison |
---|---|---|---|
exsH | 4 | 12↓c | expR+sinI mutant vs expR+sinI+ |
exsH | 5 | 11.2↓ | expR mutant sinI+ vs expR+sinI+ |
ndvA | 2 | 5.9↑ | expR mutant sinI mutant vs expR+sinI mutant |
ndvB | 0.1 | 0.6 | expR mutant sinI mutant vs expR+sinI mutant |
flaF | 3.5 | 7.1↓ | expR mutant sinI mutant vs expR+sinI mutant |
cheY1 | 3.2 | 7.6↓ | expR mutant sinI mutant vs expR+sinI mutant |
The fold change is based on the M value from the microarray data. Refer to Table 3.
The fold change is calculated from the Ct values obtained in the real-time PCR analyses. The experiments include 16S RNA multiplexed as an internal control.
The arrow indicates the direction of expression in the strain listed first in the “relevant comparison” column.