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. 2016 Jun 15;6:27768. doi: 10.1038/srep27768

Figure 3. Rex affected influenza viral RNA and protein synthesis.

Figure 3

MDCK cells were infected with influenza virus A/WSN/33 (MOI = 0.1) in the presence of Rex (25 μg/mL) at −1 to 0 h. (a) Schematic diagram of Rex treatment. The cells were collected for analysis by RT-qPCR (b), western blotting (c), and immunofluorescence microscopy (d). (b) viral RNA expression was detected by qPCR using specific primers for M1. A representative result is shown based on two reproducible experiments, where each condition was performed in triplicate. (c) The viral protein expression levels were determined by immunoblotting. Cell lysates (30 μg/lane) were subjected to SDS-PAGE and western blotting using antibodies against viral proteins (HA, NP, and M1) and an internal control (GAPDH). A representative result is shown based on three experiments. (d) Indirect immunofluorescence was evaluated using specific NP antibodies. Cellular nuclei were stained with Hoechst dye. A representative result is shown based on three experiments.