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. 2016 Jun 15;6:27768. doi: 10.1038/srep27768

Figure 6. Rex may suppress HA-mediated fusion.

Figure 6

(a,b) Rex inhibited HA-mediated haemolysis. Influenza virus A/WSN/33 was treated with serially diluted Rex, and guinea pig RBCs were added. Virus-induced haemolysis was measured at 540 nm. The results are shown as the percentage inhibition. Tertiary butylhydroquinone (TBHQ) is a HA-mediated haemolysis inhibitor and it was used as a positive control36. (b) The total protein content in the supernatants was determined using the Bradford method. (c) HA-mediated cell–cell fusion was detected using Giemsa solution, and multinucleated giant cells (shown by arrowheads) were observed by microscopy. The number of multinucleated giant cells was counted in 10 randomly selected fields for each treatment. The results represent the means ± SD based on three independent experiments.