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Expression of the Nox family proteins in cultured vascular mural cells. (a) PCR analysis of the Nox family proteins and 18 s rRNA in cultured brain microvascular pericytes and SMCs. (b) Relative gene expression of Nox4 mRNA at baseline and at 1% hypoxia for 24 and 48 hours in cultured pericytes and SMCs. Values are mean ± SD (n = 3, *p < 0.05). (c) Effects of reoxygenation for 0, 2, 6, and 24 hours on the hypoxia-induced Nox4 upregulation. Values are mean ± SD (n = 3, *p < 0.05).
