Figure 3.

Ventral periaqueductal gray/dorsal raphe (vPAG/DR) tyrosine hydroxylase (TH) neurons project to bed nucleus of the stria terminalis (BNST) and central nucleus of the amygdala (CeA), but not rostral ventral lateral medulla (RVLM). In a TH-cre mouse line, a cre-inducible enhanced yellow fluorescent protein (eYFP) viral construct was injected into the vPAG/DR (a), and a cre-inducible mCherry virus into the VTA. eYFP-expressing vPAG/DR TH terminals were observed in the BNST (b) and the CeA (c). In addition, when a retrograde bead injection was infused in the RVLM, neurons in the vPAG/DR that project to RVLM did not co-localize with TH (d).