Table 4.
Commercially available, chemically modified matrices for target immobilization for small molecule aptamer selections.
| Chemical moiety of ligand | Matrix chemistry | Magnetic beadA,E | Agarose |
|---|---|---|---|
| COOH CHO | hydrazide | Adipic acid dihydrazide AgaroseC | |
| COOH CHO (EAH Sepharose only) | amine | M-270 Amine | EAH Sepharose 4BB Affi-Gel 102 GelD CH sepharose 4BC CarboxyLink Coupling ResinE |
| NH2 | aldehyde | AminoLink Plus Coupling ResinE AminoLink Coupling ResinE | |
| NH2 | cyanogen bromide | CNBr-Activated Sepharose 4BB CNBr-Activated Sepharose 6 MBB | |
| NH2 | N-hydroxy succinimide (NHS) | NHS-Activated Sepharose E Fast FlowB Affi-Gel 10D Affi-Gel 15D Pierce NHS-Activated AgaroseE | |
| NH2 N-nucleophiles | carbonyl diimidazole | Pierce CDI-activated Agarose ResinE | |
| NH2 SH | tosylactivated | M-280 Tosylactivated MyOne Tosylactivated | |
| NH2 SH (Dynabeads only) | carboxylic acid | M-270 Carboxylic Acid MyOne Carboxylic Acid | ECH Sepharose 4BB |
| NH2 SH OH (Sepharose only) | epoxy | M-270 Epoxy | Epoxy-Activated Sepharose 6BB |
| SH | iodacetyl | SulfoLink Coupling ResinE | |
| SH heavy metal ions alkyl and aryl halides addition to C=O C=C N=N | thiol | Activated Thiol Sepharose 4BB Thiopropyl Sepharose 6BB |
Manufacturers are indicated by superscripted capitals.
A
brand name Dynabeads.
B
GE Healthcare.
C
Sigma.
D
Bio-Rad.
E
ThermoFisher Scientific.