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Primer extension analysis of surE in G. sulfurreducens DL1 (lanes 1 and 3) and rpoS mutant DLCN16 (lanes 2 and 4). Total RNA was extracted from mid-log (lanes 1 and 2) or stationary-phase (lanes 3 and 4) cultures. The transcription initiation site P1 is indicated. (B) DNA sequence of the surE regulatory region. The arrow indicates the transcription start point.
