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Purification of citramalate synthase from E. coli BL21(DE3) carrying pEX_2350
| Purification step | Vol (ml) | Total protein (mg) | Total activity (nmol/min) | Sp act (nmol/min/mg of protein) | Yield (%) | Fold purification |
|---|---|---|---|---|---|---|
| Crude extract | 4.00 | 11.3 | 1,996 | 176.6 | 100 | 1.0 |
| Six-His tagged | 0.50 | 0.2 | 505.5 | 2,527.5 | 25.3 | 14.3 |
