TABLE 5.

Substrate specificity of purified L. interrogans citramalate synthase^a

Substrate	Product formedb	Activity compared with that of pyruvate (%)
Pyruvate	0.455	100
α-Ketoisovalerate	0	0
α-Ketobutyrate	0.003	0.66
α-Ketoisocaproate	0	0
α-Ketoglutarate	0	0

^aThe concentration of each substrate was 1 mM in the final assay mixture. All of the assay conditions are described in Materials and Methods.

^bValues are optical densities at 412 nm.