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. 2016 Jun 15;2:12. doi: 10.1186/s40851-016-0047-2

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Changes in blood osmolality (a) and plasma Na⁺ (b) and Cl⁻ (c) concentrations after direct SW transfer of SW-experienced medaka (solid circles) and control FW medaka (open circles). Values are means ± SEM. Numerals in parentheses indicate the number of samples examined. Different letters indicate significant differences within the same group (ANOVA, Tukey-Kramer multiple comparison test, P < 0.05). Asterisks indicate significant differences between the two groups at the same time points (two-sided Student’s t test, *P < 0.05 and **P < 0.01)