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. 2016 Jun 14;47:64. doi: 10.1186/s13567-016-0346-9

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Detection of serum antibodies in immunized pigs by blocking ELISA. Five groups of pigs (n = 4) were immunized and challenged as described in the “Materials and methods” section. Serum samples were taken at 21 days post-immunization and 0 (7 days post-booster immunization), 3, 6, 9 and 12 days post-challenge and tested for CSFV-specific NAbs. Standard deviations were shown as error bars. *Significant difference between Groups B and C (P < 0.05); **very significant difference between Groups B and C (P < 0.001); CFU: colony forming units.