Table 3.
Detection of viral RNA in whole blood samples from immunized pigs after virulent challenge by real-time RT-PCR
| Groups | Pig No. | Days post-challenge | ||||
|---|---|---|---|---|---|---|
| 0 | 3 | 6 | 9 | 12 | ||
| A: rAdV-SFV-E2 (106 TCID50) | A1 | – | – | – | – | – |
| A2 | – | – | – | – | – | |
| A3 | – | – | – | – | – | |
| A4 | – | – | – | – | – | |
| B: rAdV-SFV-E2 (105 TCID50) + BG (1010 CFU) | B1 | – | – | – | – | – |
| B2 | – | – | – | – | – | |
| B3 | – | – | – | – | – | |
| B4 | – | – | – | – | – | |
| C: rAdV-SFV-E2 (105 TCID50) | C1 | – | – | – | – | – |
| C2 | – | – | 1.49 × 104 | 3.70 × 103 | / | |
| C3 | – | – | – | – | – | |
| C4 | – | – | 2.70 × 103 | 4.40 × 103 | 1.97 × 102 | |
| D: BG (1010 CFU) | D1 | – | – | 2.35 × 104 | 5.04 × 104 | / |
| D2 | – | – | 4.86 × 104 | 2.43 × 104 | 2.55 × 105 | |
| D3 | – | – | 5.04 × 105 | 6.96 × 105 | / | |
| D4 | – | – | 3.44 × 104 | 6.81 × 103 | 1.68 × 104 | |
| E: DMEM | E1 | – | – | 1.31 × 104 | 2.67 × 104 | / |
| E2 | – | – | 4.21 × 105 | 4.70 × 104 | 9.08 × 104 | |
| E3 | – | – | 9.76 × 104 | 6.55 × 105 | / | |
| E4 | – | – | 3.06 × 105 | 3.92 × 103 | / | |
Five groups of pigs (n = 4) were immunized and challenged as described in the “Materials and methods”. Whole blood samples were collected at days 0, 3, 6, 9 and 12 post-challenge. CSFV RNA was extracted and quantified by a real-time RT-PCR described previously [17].
– not detectable, / died, CFU: colony forming units.