TABLE 1.
Plasmids used in this study
| Designation | Description | Reference |
|---|---|---|
| pUC19 | Aprorif1 lacZ | 57 |
| SuperCos 1 | Cosmid vector, Apr | Stratagene |
| pCos3Y20 | Cosmid clone, insert contains the fimNissle 1917 gene cluster, Apr | This study |
| pCos9YB4 | Cosmid clone, insert contains the iuc gene cluster and sat of strain Nissle 1917, Apr | This study |
| pCos2YE4 | Cosmid clone, insert overlaps with pCos9YB4 and contains ihaNissle 1917, Apr | This study |
| pCos2RF2 | Cosmid clone, insert overlaps with pCos2YE4 and contains the K5-specific kps gene cluster of strain Nissle 1917, Apr | This study |
| pCos3YE4 | Cosmid clone, insert contains the mch-mcm, foc and iro gene clusters of strain Nissle 1917, Apr | This study |
| pCos2RA4 | Cosmid clone, insert overlaps with pCos3YE4, Apr | This study |
| pCos1YA7 | Cosmid clone, insert contains argW of strain Nissle 1917, Apr | This study |
| pPSD1917 | 7-kb EcoRI/HindIII fragment from pCos9YB4 containing satNissle 1917 subcloned in EcoRI/HindIII-digested pUC19, Apr | This study |