Fig. 5.

Effects of VYE on LPS-induced (a) translocation of NF-kB p65 into the nucleus and (b) phosphorylation of IkBα in RAW 264.7 cells. Cells were pretreated with VYE for 1 h and stimulated with LPS for a further (a) 1 h or (b) 30 min. β-actin and TATA box-binding protein (TBP) were used as control proteins of the cytosolic and nuclear fractions, respectively. The experiment was repeated three times independently, and similar results were obtained