Figure 1.

CDH1 D-HRMA graphs using serial dilutions of methylated DNA (from 100 to 0%) (a) The amplification plots were obtained for all standard dilutions (from 100 to 0% methylated DNA) as the template, with comparable Ct values; (b) The normalized fluorescence HRM profiles of various amplicons, amplified from each standard diluted methylated DNA; (c) The differential fluorescence plots were obtained by normalizing the HRM profiles against the unmethylated DNA; (d) The melting curve of the standard dilutions identified the specificity of the assay