TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Genotype | Reference or source |
|---|---|---|
| Strains | ||
| AB2847 | aroB malT tsx thi | 6 |
| IS1031 | fecA aroB thi malT tsx fepA lac::Tn10 | 24 |
| AA93 | F− Δfec araD139 ΔlacU169 rpsL150 relA1 flbB5301 deoCI ptsF25 rbsR thi aroB | 38 |
| Plasmids | ||
| pT7-7 | ori ColE1 phage T7 gene promoter Ampr | 44 |
| pMON37 | fecBCDE on pT7-7 | 39 |
| pASA29 | pASA32 with SphI and BsiWI restriction sites | This study |
| pASA30 | pT7-7 fecA Δ313-328 | This study |
| pASA31 | pT7-7 fecA Δ423-433 | This study |
| pASA32 | pT7-7 fecA Δ517-533 | This study |
| pASA33 | pT7-7 fecA Δ519-533 V518G | This study |
| pASA34 | pT7-7 fecA Δ563-577 F562P | This study |
| pASA35 | pT7-7 fecA Δ568-577 D567P | This study |
| pASA36 | pT7-7 fecA Δ616-630 | This study |
| pASA37 | pT7-7 fecA Δ662-673 | This study |
| pASA38 | pT7-7 fecA Δ713-727 | This study |
| pASA39 | pT7-7 fecA Δ517-533 Δ563-577 F562P | This study |
| pASA40 | pT7-7 fecA T138A | This study |
| pASA41 | pT7-7 fecA R365A | This study |
| pASA42 | pT7-7 fecA R380A | This study |
| pASA43 | pT7-7 fecA R438A | This study |
| pASA44 | pT7-7 fecA Q570A | This study |
| pASA45 | pT7-7 fecA D573A | This study |
| pASA46 | pT7-7 fecA K525A | This study |
| pASA47 | pT7-7 fecA R578A | This study |
| pASA48 | pT7-7 fecA E541A | This study |
| pASA49 | pT7-7 fecA E541R | This study |
| pASA50 | pT7-7 fecA E587A | This study |
| pASA51 | pT7-7 fecA E587R | This study |
| pASA52 | pT7-7 fecA R150A E541R | This study |
| pASA53 | pT7-7 fecA R150A E587R | This study |
| pT7-6 | ori ColE1 phage T7 gene promoter, Ampr | 44 |
| pHK763 | pT7-6 fhuA | 27 |
| pASA54 | pT7-7 fecA Δ516-535 replaced by fhuA 243-273 | This study |
| pASA55 | pT7-7 fecA Δ516-535 replaced by fhuA 318-339 | This study |
| pASA56 | pT7-7 fecA Δ516-535 replaced by fhuA 394-419 | This study |
| pASA57 | pT7-7 fecA Δ516-535 replaced by fhuA 502-515 | This study |
| pHSG576 | pSC101 derivative, Cmr | 45 |
| pMMO203 | pHSG576 fecIR | 38 |
| pMMO1034 | ori Col E1 AmprfecA-lacZ | 38 |
| pASA58 | pMMO203 fecA Δ313-328 | This study |
| pASA59 | pMMO203 fecA Δ423-433 | This study |
| pASA60 | pMMO203 fecA Δ517-533 | This study |
| pASA61 | pMMO203 fecA Δ519-533 V518G | This study |
| pASA62 | pMMO203 fecA Δ563-577 F562P | This study |
| pASA63 | pMMO203 fecA Δ568-577 D567P | This study |
| pASA64 | pMMO203 fecA Δ616-630 | This study |
| pASA65 | pMMO203 fecA Δ662-673 | This study |
| pASA66 | pMMO203 fecA Δ713-727 | This study |
| pASA67 | pMMO203 fecA Δ517-533 Δ563-577 F562P | This study |
| pASA68 | pMMO203 fecA T138A | This study |
| pASA69 | pMMO203 fecA R365A | This study |
| pASA70 | pMMO203 fecA R380A | This study |
| pASA71 | pMMO203 fecA R438A | This study |
| pASA72 | pMMO203 fecA Q570A | This study |
| pASA73 | pMMO203 fecA D573A | This study |
| pASA74 | pMMO203 fecA K525A | This study |
| pASA75 | pMMO203 fecA R578A | This study |
| pASA76 | pMMO203 fecA E541A | This study |
| pASA77 | pMMO203 fecA E541R | This study |
| pASA78 | pMMO203 fecA E587A | This study |
| pASA79 | pMMO203 fecA E587R | This study |
| pASA80 | pMMO203 fecA R150A E541R | This study |
| pASA81 | pMMO203 fecA R150A E587R | This study |
| pASA82 | pMMO203 fecA Δ516-535 replaced by fhuA 243-273 | This study |
| pASA83 | pMMO203 fecA Δ516-535 replaced by fhuA 318-339 | This study |
| pASA84 | pMMO203 fecA Δ516-535 replaced by fhuA 394-419 | This study |
| pASA85 | pMMO203 fecA Δ516-535 replaced by fhuA 502-515 | This study |