TABLE 1.
Primers used to amplify tra sequences of bait plasmids
| Bait construct | Primersa (5′ primer, 3′ primer) | tra ntb |
|---|---|---|
| pAS1traH | GCAGCGTCcatGGATGTGAACAGCG, CGTCCAGGaTCCCATAAAGGCG | 8090-8115, 19486-19466 |
| pAS1traF | CCGGCGccatgGGAAAGATGCAGGC, CATAAATCAggATCCGCGATTAAAAATTGG | 15127-15150, 15843-15815 |
| pAS1traW | GGAACCATGggATGCCGGG, CAGCCACAGgaTgCGCTTCAT | 10726-10742, 11381-11361 |
| pAS1traU | CAGAGGAGGcAtGgAAATGAAGC, GCTTCATCATggAtCCTCACAGGA | 11345-11374, 12368-12346 |
| pAS1trbl | TACAGGAGccaTgGGCATGAGTTC, GCCCCCGGATCcTCATGGTTC | 10335-10356, 10747-10727 |
a
Lowercase letters indicate mutations that were introduced to aid cloning.
b
tra sequence coordinates were obtained from reference 15.