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Density around Met54 of VP3 in the O1Manisa variant, showing breakage of main-chain density in the twinned structure (a) and recovery of main-chain density in the detwinned structure (b). Similarly, twinned density (c) and detwinned density (d) is shown around residue Gln133 of VP1. Phases were derived from refinement with PHENIX in both maps. Density is drawn at a σ of 1.0.
