Table 2.
Cellular PLFA profiles comparison of the strain BFH1 and other phylogenetically related methanotrophic genera or species.
| Fatty acids | 1 | 2 | 3 | 4 | 5 |
|---|---|---|---|---|---|
| C13:1 | 0.27 | ||||
| iC14:0 | 0.59 | 1.24 | |||
| C14:0 | 0.78 | 1.97 | 0.8–6.2 | 15.8 | |
| C14:0 2-OH | 0.33 | ||||
| C15: | 0.80 | 3.51 | 2.07 | 0–1.7 | 1.56 |
| C15:1ω8 | 0.22 | ||||
| C15:1ω6 | 0.12 | 0.17 | |||
| iC16:0 | 0.26 | ||||
| C16:1ω11 | 5.46 | ||||
| C16:1ω7c | 13.83 | 3.46 | 10.6–23.1 | 47.3 | |
| C16:1ω6c | 3.9–12.3 | 8.03 | |||
| C16:1ω5c | 0.37 | 3.2–9.0 | |||
| C16:1ω5t | 1.8–6.0 | ||||
| C16:0 | 54.38 | 63.67 | 37.24 | 33.5–56.0 | 19.6 |
| C16:1 | 11.90 | ||||
| C16:0 3-OH | 0.28 | 0.64 | 1.78 | ||
| 9-o-Me-C16:0 | 4.62 | ||||
| C17:0cyc | 26.33 | 8.99 | 4.71 | 0–14.0 | |
| aC17:0 | 0.68 | ||||
| C17:0 | 0.46 | 0.34 | 2.52 | ||
| C17:1 | 0.43 | 0.19 | |||
| C17:1ω6 | 0.62 | 0.26 | |||
| C17:1ω7c | 0–1.9 | ||||
| 9-o-Me-C17:0 | 0.60 | ||||
| 11-0-Me-17:0 | 0.60 | ||||
| C17:0 | 0.31 | ||||
| iC18:0 | 0.26 | 0.6–1.8 | |||
| C18:0 | 0.17 | 1.74 | 0–2.1 | ||
| C18:1ω7c | 0.71 | 0.35 | 0–6.5 | ||
| C18:1ω9c | 0.62 | 35.16 | 0–2.9 | ||
| C19:0cyc | 0.6–1.8 | ||||
| C19:1cyc | 1.37 | 2.41 |
Strains: (1) Strain BFH1 (data from this study); (2), Methylocaldum sp. O-12 (Eshinimaev et al., 2004); (3) Methylothermus thermalis MYHTT (Tsubota et al., 2005); (4) Methylococcus capsulatus (data from Bowman et al., 1993); (5) Methylomagnum ishizawai RD11D-PrT (Khalifa et al., 2015). Values are given as a percentage of total fatty acids.
Significance of bold values: to compare major fatty acids among methane oxidizers.