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. Author manuscript; available in PMC: 2016 Aug 18.
Published in final edited form as: Nature. 2016 Feb 18;530(7590):358–361. doi: 10.1038/nature16955

Figure 1. MMTV intasome (Int) characterization.

Figure 1

a, Purification by SEC. Elution positions of mass standards in kDa are indicated. b, Integration assay schematic. Int or IN plus vDNA was reacted with supercoiled tDNA, which can yield half site (h.s.) or concerted integration (c.i.) products. c, Ethidium bromide-stained agarose gel. Lane 1–3 reactions were initiated with IN; vDNA was omitted from lane 1. Raltegravir (RAL) was included as indicated. Lanes 4 and 5, Int reactions. Migrations positions of h.s. products that co-migrate with open circular (o.c.) tDNA, c.i. products, supercoiled (s.c.) tDNA and mass standards in kb are indicated. For gel source data, see Supplementary Figure 1. d, Sequenced Int-mediated concerted integration products (n=35).