Table 1.
Strains, plasmids and synthetic oligonucleotide primers
| Item | Description | Reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | E. coli, α-complementation | Fermentase (USA) |
| Pichia pastoris | Protein expression host | Invitrogen (USA) |
| Saccharomyces cerevisiae | Native Inveratse source | Sigma (USA) |
| Plasmid | ||
| pPinkα-HC | For integration in P. pastoris | Invitrogen (USA) |
| Oligonucleotide primers | ||
| INV-P Forward | 5′-TCTATGACTAACGAAACTTCTG-3′ | |
| INV-P Reverse | 5′-CTTAACTTCTCTAACTTGGAAC-3′ | |
| 5′ Aox1 primer | 5′-GACTGGTTCCAATTGACAAGC-3′ | |
| 3′ Aox1 primer | 5′-GCAAATGGCATTCTGACATCC-3′ | |