Table 2.

The data were obtained from the supernatant of 200 ml culture of methanol-induced P. pastoris transformants

Steps	Invertase Activity (Units)	Total protein (mg)	Specific activity (Units/mg)	Purification (Fold)
Centrifugal supernatant of the culture	65.26	1.40	46.61	1
70 % Ammonium sulfate precipitation	35.71	0.97	36.82	0.79
Ultrafiltration	49.00	1.21	59.30	1.27
His-tag FPLC	80.89	0.453	178.56	3.83

One unit of invertase is defined as the amount of enzyme that hydrolysed sucrose to yield 1 μmol of reducing sugar/min at 50 °C and pH 4.8