TABLE 2.
Resistance and sensitivity of C. jejuni strains to agents of oxidative and nitrosative stress
| Stress inducer | Initial concn on disk | Mean diam of disk inhibition zone (mm)a ± SEM (P) for C. jejuni:
|
|||
|---|---|---|---|---|---|
| NCTC 11168 | CJCGB01 (cgb mutant) | CJA01 (ahpC mutant) | CJAC01 (ahpC cgb mutant) | ||
| Methyl viologen | 3% (wt/vol) | 37.2 ± 0.8 | 36.4 ± 0.6 (0.40) | ND | ND |
| Cumene hydroperoxide | 3% (wt/vol) | 43.9 ± 2.1 | 42.6 ± 0.7 (0.56) | 59.0 ± 0.6 (0.001*) | 62.3 ± 1.5 (0.002*) |
| Hydrogen peroxide | 3% (wt/vol) | 38.7 ± 2.5 | 37.2 ± 0.5 (0.58) | 45.0 ± 1.0 (0.16) | 40.0 ± 0.6 (0.16) |
| S-Nitrosoglutathione | 100 mM | 9.2 ± 0.9 | 18.4 ± 2.4 (0.002*) | 10.0 ± 0.6 (0.29) | ND |
a
Values are expressed as mean diameter of the zone of killing. Data shown are from nine replicates. Significant P values, where P < 0.05; are indicated by an asterisk. P values are a result of t test, assuming an equal variance (two tailed) comparing the wild type with each mutant. ND, not determined.