Table 1. Example of ancillary components for clinical CAR-T manufacturing.

Manufacturing phases	Ancillary components
T-cell selection and activation	○  Selection accessory set, such as the CliniMACS set
	○  Components for selection medium, such as X-VIVO 15, OpTimizer, human serum albumin
	○  Selection reagents, such as Dynabeads, TransAct beads, ExpAct beads, Expamers
Genetic modification	○  Viral vector, such as retroviral vector and lentiviral vector
	○  Nonviral gene modification reagents, such as DNA plasmids and mRNA
T-cell expansion	○  Components for the expansion medium, such as X-VIVO 15, IL2, cytokines
Formulation	○  Cell washing accessory sets
	○  Components in cell washing medium, such as phosphate buffered saline
	○  Components in formulation medium, such as plasmalyte buffer
Cryopreservation	○  Components used in cryopreservation medium, such as dimethyl sulfoxide