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. 2016 Jun 15;3:16013. doi: 10.1038/mto.2016.13

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Green fluorescent protein (GFP) expression increases over time after infection. GFP expression reached and sustained close to 100% expression in both HCT8 parent (dashed line, circles) and tumor-initiating cells (TIC) (solid line, squares) populations. A representative experiment at MOI 0.1 is shown (a). HCT8 parent (b) and TIC (c) cells support viral replication at MOIs of 0.01, 0.1, and 1.0, with exponentially increased viral PFUs on day of maximal burst (black bars) compared to initial doses (white bars). However, viral replication does appear to occur on a different scale between populations. In both HCT8 parent (d) and TIC (e) populations, NV1066 was cytotoxic at MOI of 0.01 (circles), 0.1 (squares), and 1.0 (triangles). Cell kill is represented as percentages of live cells compared to control untreated cells grown under identical conditions. MOI: multiplicity of infection, ratio of viral particles to cells; PFU: plaque-forming units, number of viral particles.