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. 2016 Jun 15;3:16013. doi: 10.1038/mto.2016.13

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Copyright © 2016 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Fluorescence microscopy demonstrates virus-induced green fluorescent protein (GFP) expression and presumably noninfected bystander cell apoptosis. (a) Images acquired by fluorescent and differential interference contrast confocal microscopy show a tumorsphere on post-infection day 3 following infection with an MOI of 1.0. Cells expressing GFP (green) are infected with NV1066. (b) A merged image of control, uninfected cells expressing only small amounts of Annexin V and DAPI. (c) The control DIC image of the stained tumorsphere. (d) Cy-5 tagged Annexin V-positive cells appear magenta, which indicates phosphatidylserine residues of preapoptotic cells. Cells stained blue have taken up DAPI, indicating increased cellular permeability and a later stage of apoptosis.