TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)c | Reference |
|---|---|---|
| Strainsa | ||
| DH5α | hsdR recA endA | 10 |
| SF100 | ΔompT | 2 |
| AAEC185 | Δfim | 4 |
| BL21 | OmpT− Lon− | 23 |
| ORN103 | Δfim | 22 |
| Plasmids | ||
| pMON6235Δcat | Vector; Para Ampr | 15 |
| pMJ3 | PapCHis in pMON6235Δcat | 35 |
| pAP3 | PapCΔ2-11 in pMJ3 | This study |
| pMO1 | PapC E2A in pMJ3 | This study |
| pMO5 | PapC F3A in pMJ3 | This study |
| pMO2 | PapC N4A in pMJ3 | This study |
| pMO6 | PapC T5A in pMJ3 | This study |
| pMO3 | PapC D6A in pMJ3 | This study |
| pMO7 | PapC V7A in pMJ3 | This study |
| pMO8 | PapC L8A in pMJ3 | This study |
| pTN5 | PapC C70A in pMJ3 | This study |
| pTN6 | PapC C97A in pMJ3 | This study |
| pTN7 | PapC C70A + C97A in pMJ3 | This study |
| pHG81 | TnTAP insertion after PapC residue 809 in pMJ3 | —b |
| pHG102 | TnTAP insertion after PapC residue 30 in pMJ3 | —b |
| pHG105 | TnTAP insertion after PapC residue 124 in pMJ3 | —b |
| pHG133 | TnTAP insertion after PapC residue 138 in pMJ3 | —b |
| pHG132 | TnTAP insertion after PapC residue 164 in pMJ3 | —b |
| pHG118 | TnTAP insertion after PapC residue 194 in pMJ3 | —b |
| pHG4 | TnTAP insertion after PapC residue 236 in pMJ3 | —b |
| pTN2 | PapC30NHis in pMON6235Δcat | This study |
| pTN3 | PapC124NHis in pMON6235Δcat | This study |
| pTN20 | PapC138NHis in pMON6235Δcat | This study |
| pTN19 | PapC164NHis in pMON6235Δcat | This study |
| pTN4 | PapC194NHis in pMON6235Δcat | This study |
| pTN18 | PapC236NHis in pMON6235Δcat | This study |
| pMMB91 | Vector; Ptac Kanr | 8 |
| pKD101 | PapC in pMMB91 | 8 |
| pAN2 | FimD in pMMB91 | 28 |
| p10ND | FimDΔ3-12 in pAN2 | This study |
| pNS | PapC10-FimD in pAN2 | This study |
| pNaeIS | PapC25-FimD in pMMB91 | This study |
| pNarIS | PapC180-FimD in pMMB91 | This study |
| pMJ2 | ΔpapC pap operon Ptrc Tetr | 35 |
| pPAP58 | PapDJKEFG Ptac Kanr | 13 |
| pJP1 | PapDG Ptac Kanr | 8 |
| pLS101 | PapD Ptac Kanr | 29 |
| pETS6 | Δfim fim operon natural promoter Clmr | 28 |
| pETS8 | FimC Ptac Ampr | 26 |
| pETS1007 | FimCH Ptac Ampr | 27 |
a
All strains are E. coli K-12, except BL21, which is E. coli B.
b
—, C. Martin and D. Thanassi, unpublished data.
c
Ampr, ampicillin resistance; Kanr, kanamycin resistance; Clmr, chloramphenicol resistance; Tetr, tetracycline resistance; Para, arabinose-inducible promoter, Ptac or Ptrc, IPTG-inducible promoter.