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. 2016 Jun 15;11(6):e0156800. doi: 10.1371/journal.pone.0156800

Fig 3. IgG1 and IgG4 variants of anti-Dsg3 PV mAbs are pathogenic and cause suprabasal blisters in human skin.

Fig 3

(A) 25 μg anti-Dsg3 PV IgG1 or IgG4 was injected ex vivo into human skin sections and incubated for 18 hours prior to harvest for histologic analysis. IgG1 and IgG4 of three anti-Dsg3 PV mAbs, F706, F779 and Px43 induced suprabasal blisters in human skin. PBS/ETA served as negative control. Scale bar, 100 microns. (B) The extent of histologic blistering caused by IgG1 and IgG4 mAb variants is comparable. (C) DIF staining of human skin with PV isotype mAbs illustrates cell surface binding in skin epidermis following injection of mAbs. D: dermal part of skin. Scale bar, 20 microns.