Fig 2. Effect of dabigatran, argatroban, or DAPA on thrombin binding to immobilized γA/γA-fibrin, γA/γ′-fibrin, or factor Va.
Increasing concentrations of dabigatran (closed circles), argatroban (closed triangles), or DAPA (closed squares) up to 5 μM were pre-incubated with thrombin prior to injection into individual flow cells containing immobilized (A) γA/γA-fibrin, (B) γA/γ′-fibrin, or (C) factor Va. Sample injection was followed by a 600 s wash with buffer to monitor dissociation. Left panels; representative sensorgrams of the effects of increasing concentrations of dabigatran, argatroban or DAPA on thrombin binding are shown in succession. Right panels; the ratios of Req values of thrombin bound in the absence (Boundo) and presence (Bound) of dabigatran, argatroban or DAPA are plotted against the input inhibitor concentrations. Open symbols represent the effects of the inhibitors on FPR-thrombin binding to γA/γA-fibrin, γA/γ′-fibrin, or factor Va. Data points represent the mean ± S.D. of 3 experiments, and the lines represent nonlinear regression analysis.