Fig 5. Effect of dabigatran, argatroban, DAPA, or hirudin on the dissociation of ¹²⁵I-thrombin from fibrin clots.

Clots were formed around plastic loops by incubating 3 μM γ_A/γ_A-fibrinogen and 30 nM factor XIII with 10 nM ¹²⁵I-thrombin in the presence of 2 mM CaCl₂ for 1 h. Clots were immersed in 10 ml TSTwCa buffer alone, or buffer containing 100 nM dabigatran, argatroban, or DAPA, or 2 M NaCl. At intervals, aliquots were removed and counted for radioactivity. Residual counts were normalized with respect to time zero and plotted versus time. Data were analyzed by two phase exponential decay (lines) to determine the half-lives of dissociation. Symbols represent the mean ± SD of 4 experiments.