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Stx2 coprecipitates with FI-29 LPS. LPS from FI-4 and FI-29 alone, Stx2 from an induced culture of C600::933W, or Stx2 from the induced culture with LPS from FI-4 or FI-29 was centrifuged at 150,000 × g. The presence of Stx2 in the pellets was detected by Western analysis with anti-Stx2 monoclonal antibody 11E10. Stx2 (10 μg) was loaded onto the gel as a positive control for Stx2 detection.
