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. 2016 Jun 15;11(6):e0157593. doi: 10.1371/journal.pone.0157593

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© 2016 Huang et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Gel-filtration chromatography was carried out by the AKTA-FPLC system. The column was calibrated with proteins of known molecular weight: thyroglobulin (670 kDa), γ-globulin (158 kDa), ovalbumin (44 kDa), and myoglobin (17 kDa). The K_av values for the standard proteins and the SaDnaD variants were calculated from the equation: K_av = (V_e − V_o)/(V_c − V_o), where V_o is the column void volume, V_e is the elution volume, and V_c is the geometric column volume. A standard linear regression curve was generated by plotting the log of the molecular mass of the calibration proteins against their K_av values.