Table 2. Primers used for construction of GST plasmids.

Oligonucleotide	Primer
GST-SaDnaD-EcoRI-N	GGGGAATTCGATAAATATCAATTAAAA
GST-SaDnaD-XhoI-C	GGGCTCGAGTTACTTACCATCAAGGTT
GST-SaDnaD1-195-EcoRI-N	GGGGAATTCGATAAATATCAATTAAAA
GST-SaDnaD1-195-XhoI-C	GGGCTCGAGTTAAGAATCGTCAATTGT
GST-SaDnaDY176A-N	AGCTTTAAAGCTATGGATCGTATTTTA
GST-SaDnaDY176A-C	ACGATCCATAGCTTTAAAGCTAAGTTT

These plasmids were verified by DNA sequencing. Underlined nucleotides indicate the designated site for the restriction site or the mutation site.