Table 3. Morbidity and mortality parameters measured in animals subjected to four separate challenge experiments (Exp.1, 2, 3 and 4)*.
| Parameters | Group I (n = 10) | Group II (n = 10) | Group III (n = 10) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Exp. 1 | Exp. 2 | Exp.3 | Exp. 4 | Exp. 1 | Exp. 2 | Exp.3 | Exp. 4 | Exp. 1 | Exp. 2 | Exp.3 | Exp. 4 | |
| Morbidity (%) | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 | 100 |
| Mean duration for clinical onset (days) (range) | 2.7(1–5) | 4.2(1–6) | 3 (1–5) | 3.4(2–4) | 3.1(2–4) | 3.4(2–5) | 3(2–5) | 2.9(2–4) | 1.1(1–3) | 1.2(1–2) | 1.3(1–2) | 1.5(1–2) |
| Overall mean patent period (days) (range) | 10.1(7–12) | 6(6–12) | 10.1(8–12) | 10.1(9–12) | 7(5–8) | 9.2(4–12) | 9.7(6–13) | 9.2(7–12) | 7 | 6 | 8.76–13) | 11.3(9–13) |
| Mean patent period (days) for birds that died (range) | 7 | 7.5(7–8) | 11(9–13) | 12 | 7(6–8) | 5.3(4–6) | 8 | 12 | 7 | 6 | 8.7(5–13) | 11.3(9–13) |
| Mean patent period (in days) for birds that survived (range) | 10.9(10–12) | 5.6(4–8) | 9.9(9–11) | 9.9(9–11) | 7(5–8) | 10.9(9–12) | 10.1(8–12) | 8.9(7–12) | NA | NA | NA | NA |
| Mortality (%) | 20 | 20 | 10 | 10 | 50 | 30 | 20 | 10 | 100 | 100 | 100 | 100 |
| Total no. sick birds but recovered (%) | 80 | 80 | 90 | 90 | 50 | 70 | 80 | 90 | 0 | 0 | 0 | 0 |
| Case fatality rate (%) | 20 | 20 | 10 | 10 | 50 | 30 | 20 | 10 | 100 | 100 | 100 | 100 |
| Mean death time (MDT) | 8 | 12 | 14 | 14 | 7.8 | 9.3 | 10 | 14 | 8.2 | 8 | 9.8 | 12.8 |
| Protection (%) | 80 | 80 | 90 | 90 | 50 | 70 | 80 | 90 | 0 | 0 | 0 | 0 |
*Experiments 1, 2, 3 and 4 were carried out on birds of 21, 28 and 35 and 42 days of age. In both cases, Group 1 (vaccinated with rHVT-H5 vaccine at 1 day old), Group II (vaccinated with inactivated KV-H5 vaccine at 8 days old), Group III (unvaccinated control). All birds were challenged with HPAI H5N1 virus (A/Chicken/Egypt/128S/2012 (H5N1)) (GeneBank Accession No. JQ858485) via the oculo-nasal route with 100 μl (50_ul in eye, 50_ul in nose) of inoculum containing 106 EID50 per duck.