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. 2016 May 23;72(Pt 6):427–433. doi: 10.1107/S2053230X16006920

Table 1. Macromolecule-production information.

Source organism T. kodakarensis KOD1
DNA source T. kodakarensis genomic DNA
Forward primer GGGGCTGCAGCATATGCTCTTTGGAATAGGCCTC
Reverse primer AAAAGGATCCTACTCCCTCACCCACAGCG
Cloning vector pET-21a(+)
Expression vector pET-21a(+)
Expression host E. coli Rosetta2(DE3)pLysS
Complete amino-acid sequence of the construct produced MLFGIGLMPHGNPALSPEDKETEKLAGVLKDIGKAFSDADSYVLISPHNVRISDHLGVIMAQHLISWLGFEGVELPGEWETDRGLAEEVYNAWKGAEIPTVDLHFASRSGRYSRWPLTWGELIPLQFLEKKPLVLLTPARRLSRETLIKAGEVLGEVLEGSEKKIALIVSADHGHAHDENGPYGYRKESEEYDRLIMELINESRLEELPEIPDELIEKALPDSYWQMLIMLGAMHRVPVKLVESAYACPTYFGMAGALWVRE

The restriction sites used in the primer sequences are underlined.