Table 1. Macromolecule-production information.
Source organism | T. kodakarensis KOD1 |
DNA source | T. kodakarensis genomic DNA |
Forward primer† | GGGGCTGCAGCATATGCTCTTTGGAATAGGCCTC |
Reverse primer† | AAAAGGATCCTACTCCCTCACCCACAGCG |
Cloning vector | pET-21a(+) |
Expression vector | pET-21a(+) |
Expression host | E. coli Rosetta2(DE3)pLysS |
Complete amino-acid sequence of the construct produced | MLFGIGLMPHGNPALSPEDKETEKLAGVLKDIGKAFSDADSYVLISPHNVRISDHLGVIMAQHLISWLGFEGVELPGEWETDRGLAEEVYNAWKGAEIPTVDLHFASRSGRYSRWPLTWGELIPLQFLEKKPLVLLTPARRLSRETLIKAGEVLGEVLEGSEKKIALIVSADHGHAHDENGPYGYRKESEEYDRLIMELINESRLEELPEIPDELIEKALPDSYWQMLIMLGAMHRVPVKLVESAYACPTYFGMAGALWVRE |
The restriction sites used in the primer sequences are underlined.