Table 1. Summary of cloning and the construct produced.

The NcoI and BamHI restriction sites are underlined in the forward and reverse primers, respectively. The additional N-terminal residues of the tag, encoding a hexahistidine tag and a Tobacco etch virus (TEV) protease cleavage site, are underlined in the amino-acid sequence of the construct produced.

Source organism	T. africanus strain TCF52B
DNA source	Genomic
Forward primer	5′-CATGCCATGGCTATGAAGATAGAATACTTTAAG-3′
Reverse primer	5′-CGGGATCCTTATTCTTTATTACTTATTTCTAGG-3′
Cloning vector	pEHISTEV
Expression vector	pEHISTEV
Expression host	E. coli BL21-CodonPlus-RIL
Complete amino-acid sequence of the construct produced	MSYYHHHHHHDYDIPTTENLYFQGAMAMKIEYFKNDKCSVCKAMLPKIQTIAKNFDIDIEVIDVIENPSYPAQKLVFTVPTVIILDKEFEIKRFARNFSISEVINTIERYLEISNKE