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. 2016 May 23;72(Pt 6):443–447. doi: 10.1107/S2053230X16007214

Table 2. Summary of the optimum crystallization conditions.

Method Microbatch under oil
Temperature (K) 289
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 50 mM Tris pH 8, 150 mM NaCl, 1 mM DTT
Composition of reservoir solution 0.1 M bis-tris pH 6.5, 0.2 M MgCl2, 25%(w/v) PEG 3350
Volume and ratio of drop 2.4 µl (1:1)