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. 2016 May 23;72(Pt 6):473–479. doi: 10.1107/S2053230X16007512

Figure 1.

Figure 1

Expression, purification and crystallization of I-SceI. (a) SDS–PAGE gel showing the degree of purity of the protein samples. (b) MALDI of I-SceI showing a major peak at 29 181.797 Da. A small peak at 14 541.306 Da arising from the doubly charged molecule can be also observed. (c) Protein secondary structure (left panel) and thermal stability (right panel) analysed by circular dichroism. (d) SEC-MALLS data of the I-SceI protein preparation. (e) Band-shift assay. The DNA target was incubated with and without I-SceI protein for 1 h on ice. The mixture was separated on a native polyacrylamide gel. The arrow indicates the band corresponding to free DNA target.