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. 2016 May 23;72(Pt 6):480–484. doi: 10.1107/S2053230X16007573

Figure 1.

Figure 1

(a) Size-exclusion chromatogram of the Os01T0156300 protein using a Superdex 75 column. The estimated MW was 31.4 kDa. (b) Size distribution of 2 mg ml−1 Os01T0156300 protein using a Zetasizer Nano ZS at 293 K. The estimated MW was 56.1 ± 15.6 kDa. (c) Anion-exchange chromatogram of the Os01T0156300 protein. (d) SDS–PAGE after anion-exchange chromatography. Elution volumes are shown for the first (12–15 ml) and second (16–18 ml) peaks of the anion-exchange chromatogram. Lane M contains molecular-weight markers (labelled in kDa).