Table 1. Macromolecule-production information.

The underlined amino-acid sequence indicates the glutathione S-transferase (GST) tag. The HRV 3C protease cleavage sites are between the Q and G residues shown in italics.

Source organism	O. sativa
Expression vector	pCold GST
Expression host	E. coli Rosetta (DE3)
Complete amino-acid sequence	MNHKVHHHHHHIEGRHMSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLF QG PGHMMAAEAWRSRFRERVVEAAERWESVGESLATALTHLKSPMHAGDEEEAAAARTRIQLAMGELVDASRNLASAMSLMKVAELLALHGGSVNPSTHLGEISLLGDQYLAERNAGIKLLEAGKDARKAYISVDGCRGNLDAILLLLDHPRVPCVDDFIEEELFVAGDNLQGAIGNAKLGTERAVGARQDVSGAN