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. 2016 May 23;72(Pt 6):480–484. doi: 10.1107/S2053230X16007573

Table 2. Crystallization of the Os01T0156300 protein.

Method Hanging-drop vapour diffusion
Plate type 24-well plates
Temperature (K) 277
Protein concentration (mg ml−1) 10
Buffer composition of protein solution 20 mM Tris–HCl pH 8.0, 1 mM DTT
Composition of reservoir solution 8% PEG 8000, 0.1 M HEPES pH 7.0, 0.2 M sodium citrate
Volume and ratio of drop 1 µl:1 µl
Volume of reservoir (ml) 0.5