Table 2. Crystallization of the Os01T0156300 protein.
Method | Hanging-drop vapour diffusion |
Plate type | 24-well plates |
Temperature (K) | 277 |
Protein concentration (mg ml−1) | 10 |
Buffer composition of protein solution | 20 mM Tris–HCl pH 8.0, 1 mM DTT |
Composition of reservoir solution | 8% PEG 8000, 0.1 M HEPES pH 7.0, 0.2 M sodium citrate |
Volume and ratio of drop | 1 µl:1 µl |
Volume of reservoir (ml) | 0.5 |