Table 3. Data collection and processing for the Os01T0156300 protein (OsPBI1).
Values in parentheses are for the outer shell.
| Native OsPBI1 | SeMet OsPBI1 | |
|---|---|---|
| Diffraction source | BL44XU, SPring-8 | BL44XU, SPring-8 |
| Wavelength (Å) | 0.90000 | 0.97894 |
| Temperature (K) | 100 | 100 |
| Detector | MX300HE | MX300HE |
| Crystal-to-detector distance (mm) | 250 | 250 |
| Rotation range per image (°) | 0.6 | 1 |
| Total rotation range (°) | 190 | 185 |
| Exposure time per image (s) | 1 | 1 |
| Space group | P21 | P21 |
| a, b, c (Å) | 89.93, 89.78, 107.09 | 90.18, 89.76, 107.92 |
| α, β, γ (°) | 90, 106.6, 90 | 90, 106.7, 90 |
| Mosaicity (°) | 0.26 | 0.43 |
| Resolution range (Å) | 50.0–1.84 (1.87–1.84) | 50.0–2.12 (2.16–2.12) |
| Total No. of reflections | 570139 | 613163 |
| No. of unique reflections | 141131 | 92035 |
| Completeness (%) | 98.0 (97.3) | 98.0 (97.3) |
| Multiplicity | 4.1 (4.2) | 4.1 (4.0) |
| 〈I/σ(I)〉 | 34.3 (3.7) | 47.8 (7.0) |
| R merge † | 0.050 (0.418) | 0.080 (0.394) |
| Overall B factor from Wilson plot (Å2) | 25.0 | 30.9 |
†
R
merge = 
, where Ii(hkl) is the ith observed intensity of reflection hkl and 〈I(hkl)〉 is the mean measurement.