Table 1. Characterization of all gene cassettes detected in the saliva metagenomic DNA from UK and Bangladesh using TDIF and MARS2 primer combination to detect the first gene cassette.
| BlastN (ORF of first gene cassette) | BlastX (ORF of first gene cassette) | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Gene cassettes /clone code | Primer pair | Size of insert including the integrase gene and first cassette (bp) | Orientation type | Distance between integrase gene and ORF of first gene cassette (bp) | Accession number | Closest homologue | Percentage identity (%) | Coverage (%) | Closest homologue | ORF size (bp) | Percentage identity (%) | Coverage (%) | The presence of ribosomal binding site | Accession number of the homologous proteins (BlastX) |
| TMB1/8/12/15 | TDIF-MARS2 | 1499 | A | 271 | KT921469 | No significant similarity | - | - | No significant similarity | 258 | - | - | Yes | - |
| TMB3/5/6/10/11/13/14/16 | TDIF-MARS2 | 1757 | A | 198 | KT921470 | Treponema putidum | 98 | 100 | Cof-like hydrolase [Treponema denticola] | 768 | 96 | 100 | Yes | WP_016512123 |
| TMB4 | TDIF-MARS2 | 1244 | B | - | KT921471 | Treponema pedis | 82 | 46 | - | - | - | - | - | - |
| TMU3/4/11 | TDIF-MARS2 | 1775 | A | 204 | KT921472 | Treponema sp. OMZ 838 | 77 | 97 | Hypothetical protein [Treponema denticola] | 777 | 99 | 100 | Yes | WP_002678613.1 |
| TMU18 | TDIF-MARS2 | 1692 | A | 161 | KT921473 | No significant similarity | - | - | Hypothetical protein [Treponema denticola] | 387 | 100 | 100 | Yes | WP_002689012.1 |