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. 2016 Jun 15;10(6):e0004754. doi: 10.1371/journal.pntd.0004754

Fig 1. MIC determination of free AmB and MFLB-AmB against liquid suspensions of Paracoccidioides brasiliensis.

Fig 1

Fungal burden as assessed by Colony Forming Units (CFU) in Paracoccidioides brasiliensis strain 18 (Pb18) (2 x 104 cells/mL) while incubated in RPMI 1640 medium at 37°C for 72 hours with AmB or MFLB-AmB, as a function of amphotericin B, or MFLB, as a function of iron concentration. After 72 hour incubation, cell suspensions were plated on BHI (brain heart infusion) media, supplemented with 4% (v/v) of horse serum, 5% (v/v) of the supernatant from the culture filtrate of isolate Pb192 and 40 mg/L gentamycin, and then incubated for five days at 37°C in a rotary shaker (220 rpm).(AmB = amphotericin B, MFLB-AmB = nanosized magnetite surface-functionalized with a bilayer of lauric acid conjugated with amphotericin B, MFLB = nanosized magnetite surface-functionalized with a bilayer of lauric acid).