Fig 3. the 72-member selectively constrained gene subnetwork is active in early brain development, particularly in the hippocampus.

A: the constrained subnetwork shows elevated signatures of preferential expression in early stages of brain development. B: the signature is most robust in the hippocampus and its ancestral structures (orange), with some enrichment in ventral forebrain and parietal cortical wall structures very early in development (8–9 post-conception weeks). C: The constrained subnetwork shows significant preferential expression in early developmental stages, with patterns of expression losing this enrichment signature by mid-gestation. Preferentially expressed nodes and the shortest paths connecting them are colored orange; grey nodes are not preferentially expressed in each displayed tissue. Overall, these data suggest the constrained subnetwork is specifically active in very early stages of hippocampal formation.