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. 2016 Jun 15;12(6):e1006109. doi: 10.1371/journal.pgen.1006109

Fig 3. NF-YB is required for proliferation of planarian SSCs and spermatogonia.

Fig 3

(A) Experimental scheme. (B) Cryosections of control and NF-YB RNAi animals stained for PH3S10 after 2 feedings and quantification of the results, showing a reduction in mitotic cells. (C) This effect is greater following 4 feedings due to the loss of spermatogonial layer. A minimum of 15 testis lobes from 7–8 animals were counted per time point. Raw data are presented in S1 Table. (D) There was little TUNEL signal in control and NF-YB(RNAi) animals after 2 feedings. (E) Following 4 feedings, the differentiated cells in knockdown animals show increased apoptosis compared to controls. See S1 Table for raw data. Scale bars, 10 μm.